Listeria is a genus of bacteria that are found in nature and in soil and is ubiquitous in the environment. There are many species of Listeria that have potential of causing disease in humans through an infection known as listeriosis. These species raise concern in the manufacturing of food. Listeria monocytogenes is an important bacterium in the genus. It is especially significant in the elderly, young, immunocompromised individuals, and pregnant woman. In rare cases, people without these risk factors can also be affected.
Listeria bacteria have been found in a variety of raw foods, such as uncooked meats, fruits, and vegetables. They have also been detected in foods that become contaminated after cooking or processing. Examples of these types of foods are soft cheeses, processed meats, and smoked seafood. Raw milk and cheeses and other foods made from unpasteurized milk may also contain the bacteria.
Listeria is killed by pasteurization and cooking; however, in some ready-to-eat foods, such as hot dogs and deli meats, contamination may occur after factory cooking but before packaging. An unusual feature of Listeria is its ability to grow in some foods even when they are at proper refrigeration temperatures.
- About 1,600 cases of listeriosis occur annually in the United States.1
- In September 2011, Listeria was identified in a specific brand of cantaloupe. As of November 2, 2011, 28 states were affected and 29 deaths were attributed to the tainted cantaloupe.1
Source: Centers for Disease Control
Atlas Listeria LSP Detection Assay
The Atlas Listeria LSP Detection Assay is a rapid, highly accurate molecular assay designed to meet the needs of the Food Safety Industry for Listeria testing. The Atlas Listeria Detection Assay is run on the Atlas® System.
The assay offers unparalleled ease of use while maintaining accuracy and sensitivity. While other tests require tedious multistep preparation processes, the Atlas Listeria Detection Assay has an easy 3-step process that gives you accurate results faster.
- Enrich—single pre-enrichment media; 24 hrs
- Transfer—no multichannel pipetting, centrifugation, or heating
- Automate—place transfer tube directly into instrument
It’s never been easier to get the results you need.
EASY 3-STEP PROCEDURE
This simple upstream processing combined with the powerful Atlas System platform provides rapid, reliable results while optimizing throughput and efficiency.
The Atlas Listeria Detection Assay harnesses the strength of three proven molecular technologies—Target Capture, TMA, and HPA—to reduce false-positives and –negatives. The result is increased sensitivity and specificity, minimizing the need for cumbersome retesting, even in the most difficult matrices.
Assay Features and Benefits
- Minimal hands-on time to reconstitute reagents
- Simple one-time reconstitution
- Extended shelf life of reagents after reconstitution
- Long stability on kit
- Kit has extended dating—manufactured with 18 months’ dating
- Provides ease in ordering
- Helps optimize inventory control
- Assures efficient use of reagents
- Easy storage of reagents
- Minimal refrigerator room required for reagent storage
- Two convenient ways to store reconstituted reagents:
- Continuous storage on instrument for 96 hours
- Once reconstituted return to refrigerator for up to 30 days’ storage
- Color-coded labels for ease in identifying reagents
- Provides ease in reconstitution
- Helps techs identity and pair reconstitution solution with reagent
- Helps assure proper placement of reagent on reagent rack
- Use of innovative docking collars for reconstitution ease
- No measuring or use of pipettes required
- Assures accurate reconstitution
- No extra equipment required
The Atlas Listeria Detection Assay is run on the Atlas System. Together, they create a completely integrated system that provides the most reliable and efficient Listeria testing available today.
Atlas System Highlights
- Automated and integrated for accuracy and reliability
- “Sample-in, result-out” design
- Automated process controls
- Full sample-to-result traceability
- Minimal hands-on time
- Fast and efficient
- Single pre-enriched commercially available media
- No secondary enrichment step required
- Provides results on prepared samples in 3 hours
- Processes 300 samples in an 8-hour work period
- Easy-to-use instrument
- Simplified one-step sample preparation
- Continuous-access sample loading
- True “walk-away” capability
- Intuitive design; little training needed
- Minimal start-up and maintenance
- Automated process controls help ensure consistency and quality
- Full sample-to-result traceability
- Positive sample identification
- Liquid level sensing
- Reagent dispense verification
- Barcoding of all samples and reagents
- Automated inventory control
Atlas Listeria Detection Assay
250 tests per kit
1. Centers for Disease Control and Prevention. Listeriosis (Listeria infection). http://www.cdc.gov/listeria/.