A rapid, accurate, and reliable molecular test for E. coli O157:H7 detection from food samples.
The Atlas® E. coli O157:H7 EG2 Detection Assay is the first AOAC-approved assay to test for the presence of Escherichia coli (E. coli) O157:H7—the E. coli strain most commonly associated with foodborne illness—in beef and produce samples. E. coli O157:H7 belongs to a serogroup of E. coli organisms known as Shiga toxin-producing E. coli (STEC), the most severe form of E. coli. The U.S. Centers for Disease Control and Prevention (CDC) estimates that there are approximately 265,000 STEC-related infections annually in the United States. More than one-third (36%) of these infections are believed to be related to O157 STEC, the most widely reported STEC to cause human illness, accounting for 96,000 foodborne illnesses annually.1
Source: Centers for Disease Control and Prevention
The Atlas E. coli O157:H7 EG2 Detection Assay is designed for the Atlas System, a fully automated molecular pathogen detection system. When used in conjunction with the Atlas System, the Atlas E. coli O157:H7 and STEC Detection Assays offer a complete platform solution for even the most rigorous food safety programs. The Atlas solution provides true “walk-away” automation with unparalleled gains in standardization and lab efficiencies, guaranteeing high-quality results and the ultimate in peace of mind.
As with other Atlas Detection Assays, the Atlas E. coli O157:H7 EG2 Detection Assay has an easy 3-step process that generates accurate results faster:
- Enrich—a single enrichment step with commercially available media allows for streamlined media preparation
- Transfer—an aliquot of the enrichment is transferred to a Roka G2 Sample Transfer Tube, with no requirement for heating, centrifugation, or sample purification
- Automate—place the Roka G2 Transfer Tube directly on the Atlas System and walk away! Final results are available in 3.5 hours
EASY 3-STEP PROCEDURE
It’s never been easier to get the results you need.
Assay Features and Benefits
The simple upstream processing power of the Atlas E. coli O157:H7 EG2 Detection Assay, combined with the powerful Atlas System, provides rapid, reliable results while optimizing throughput and efficiency. Your laboratory will reap the following benefits:
- The Atlas E. coli O157:H7 EG2 Detection Assay is offered on a single platform, with one technology for consistent, accurate results
- The assay requires minimal hands-on time to reconstitute reagents
- The ability to load samples continuously eliminates the need for sample batching, allowing the laboratory to run samples more quickly and get results faster
- First results from prepared samples are available in 3.5 hours
- The assay can process 300 samples in an 8-hour period
- The assay’s simplified test process eases lab flow
- The assay allows you to manage all your data and sample traceability through the Atlas instrument without ever having to manually input a sample ID onto the system
- The continuous-access instrument eliminates the need for batching and reagent pipetting
- Harmonized enrichment with Salmonella media aligns with the Food Safety and Inspection Service (FSIS) reference method as well as with non-proprietary media
- Roka Bioscience’s powerful molecular technology enhances the sensitivity and specificity of the Atlas E. coli O157:H7 EG2 Detection Assay
- The assay enables automated reagent tracking and sample barcoding
- An internal control is built into every reaction for the ultimate confidence in results validity
The Atlas E. coli O157:H7 EG2 Detection Assay harnesses the strength of three proven molecular technologies—Target Capture, Transcription-Mediated Amplification (TMA), and a Hybridization Protection Assay (HPA)—to reduce erroneous results. The result is increased sensitivity and specificity, minimizing the need for cumbersome retesting, even in the most difficult matrices.
Atlas E. coli O157:H7 EG2 Detection Assay
250 tests per kit
1. Centers for Disease Control and Prevention. E. coli (Escherichia coli). http://www.cdc.gov/ecoli/general/index.html.