Atlas E. coli O157:H7 EG2 Detection Assay

011401 AOAC_Ecoli

The Atlas® E. coli O157:H7 EG2 Detection Assay has been certified by the AOAC Research Institute Performance Tested MethodsSM program and assigned PTM certification #011401.


E. coli O157:H7 Overview

Escherichia coli (E. coli) O157:H7 is a gram-negative, non-spore forming, rod-shaped organism. It is a member of the Enterobacteriaceae family and may or may not be motile. The O157:H7 serotype is classified using a lipopolysaccharide to identify the O antigen, while the H is identified through a flagellar antigen. Pathogenic E. coli are categorized based on the type and severity of disease they cause.

  1. Enterotoxigenic (ETEC) – mild and brief gastroenteritis
  2. Enteropathogenic (EPEC) – mostly in infants, mild, may lead to dehydration
  3. Enteroinvasive (EIEC) – dysentery (mild)
  4. Enterohemorrhagic / Shiga Toxin-Producing (EHEC / STEC) – may cause hemolytic uremic syndrome (HUS), most severe (E. coli O157:H7 are in this serogroup)

The CDC estimates that there are approximately 265,000 STEC-related infections annually in the United States. It is further believed that 36% of these infections are related to O157 STEC infections. This E. coli serotype accounts for 96,000 foodborne illnesses annually and is the most widely reported STEC to cause human illness.1

Both the FSIS and FDA have declared E. coli O157:H7 to be an adulterant in certain raw and ready-to-eat products.

Rapid and accurate monitoring of E. coli O157:H7 is a critical need for producers of at-risk foods. This allows for unparalleled public and consumer safety with faster intervention, minimization of risk, and brand protection.

Atlas E. coli O157:H7 EG2 Detection Assay

The Atlas E. coli O157:H7 EG2 Detection Assay is a rapid, accurate, and reliable molecular test for E. coli O157:H7 detection in beef and produce samples. The assay is designed for the Atlas System—a fully automated molecular pathogen detection system.

The Atlas Solution: High-Quality Pathogen Testing Results

When used in conjunction with the Atlas System, the Atlas E. coli O157:H7 EG2 and STEC EG2 Combo Detection Assays offer a complete platform solution for even the most rigorous food safety programs. The Atlas Solution provides true “walk-away” automation with unparalleled gains in standardization and lab efficiencies, guaranteeing high-quality results and the ultimate peace of mind.

  • Simplified test process eases lab flow
  • AOAC approval for 375 g sample weight
  • Robust and not susceptible to interference from neutralizing buffers or other inhibitory substances
  • Internal control is built in to every reaction for ultimate confidence in results
  • Harmonized enrichment media with Salmonella Media alignment with the FSIS reference method or Non-proprietary media

It’s never been easier to get the results you need.


This simple upstream processing combined with the powerful Atlas System platform provides rapid, reliable results while optimizing throughput and efficiency.

Assay Technology

The Atlas E. coli O157:H7 EG2 Detection Assay harnesses the strength of three proven molecular technologies—Target Capture, TMA, and HPA—to reduce false-positives and -negatives. The result is increased sensitivity and specificity, minimizing the need for cumbersome retesting, even in the most difficult matrices.

Atlas System Highlights

  • Automated and integrated for accuracy and reliability
  • Fast and efficient, up to 300 results in an 8-h shift
  • Easy-to-use instrument
  • Automated process controls help ensure consistency and quality

Ordering Information

Atlas E. coli O157:H7 EG2 Detection Assay
250 tests per kit
Catalog #PRT-01252

Orders (or general questions about ordering): Customer Support
Product-related questions: Technical Support

1. Centers for Disease Control and Prevention. E. coli (Escherichia coli).

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