Atlas E. coli O157:H7 EG2 Detection Assay

A rapid, accurate, and reliable molecular test for E. coli O157:H7 detection from food samples.

The Atlas® E. coli O157:H7 EG2 Detection Assay is the first AOAC-approved assay to test for the presence of Escherichia coli (E. coli) O157:H7—the E. coli strain most commonly associated with foodborne illness—in beef and produce samples. E. coli O157:H7 belongs to a serogroup of E. coli organisms known as Shiga toxin-producing E. coli (STEC), the most severe form of E. coli. The U.S. Centers for Disease Control and Prevention (CDC) estimates that there are approximately 265,000 STEC-related infections annually in the United States. More than one-third (36%) of these infections are believed to be related to O157 STEC, the most widely reported STEC to cause human illness, accounting for 96,000 foodborne illnesses annually.1

Source: Centers for Disease Control and Prevention

The Atlas E. coli O157:H7 EG2 Detection Assay is designed for the Atlas System, a fully automated molecular pathogen detection system. When used in conjunction with the Atlas System, the Atlas E. coli O157:H7 and STEC Detection Assays offer a complete platform solution for even the most rigorous food safety programs. The Atlas solution provides true “walk-away” automation with unparalleled gains in standardization and lab efficiencies, guaranteeing high-quality results and the ultimate in peace of mind.

As with other Atlas Detection Assays, the Atlas E. coli O157:H7 EG2 Detection Assay has an easy 3-step process that generates accurate results faster:

  1. Enrich—a single enrichment step with commercially available media allows for streamlined media preparation
  2. Transfer—an aliquot of the enrichment is transferred to a Roka G2 Sample Transfer Tube, with no requirement for heating, centrifugation, or sample purification
  3. Automate—place the Roka G2 Transfer Tube directly on the Atlas System and walk away! Final results are available in 3.5 hours



It’s never been easier to get the results you need.


Assay Features and Benefits

The simple upstream processing power of the Atlas E. coli O157:H7 EG2 Detection Assay, combined with the powerful Atlas System, provides rapid, reliable results while optimizing throughput and efficiency. Your laboratory will reap the following benefits:


  • The Atlas E. coli O157:H7 EG2 Detection Assay is offered on a single platform, with one technology for consistent, accurate results


  • The assay requires minimal hands-on time to reconstitute reagents
    • The ability to load samples continuously eliminates the need for sample batching, allowing the laboratory to run samples more quickly and get results faster
    • First results from prepared samples are available in 3.5 hours
    • The assay can process 300 samples in an 8-hour period


  • The assay’s simplified test process eases lab flow
    • The assay allows you to manage all your data and sample traceability through the Atlas instrument without ever having to manually input a sample ID onto the system
    • The continuous-access instrument eliminates the need for batching and reagent pipetting
    • Harmonized enrichment with Salmonella media aligns with the Food Safety and Inspection Service (FSIS) reference method as well as with non-proprietary media


  • Roka Bioscience’s powerful molecular technology enhances the sensitivity and specificity of the Atlas E. coli O157:H7 EG2 Detection Assay
    • The assay enables automated reagent tracking and sample barcoding
    • An internal control is built into every reaction for the ultimate confidence in results validity

Assay Technology

The Atlas E. coli O157:H7 EG2 Detection Assay harnesses the strength of three proven molecular technologies—Target Capture, Transcription-Mediated Amplification (TMA), and a Hybridization Protection Assay (HPA)—to reduce erroneous results. The result is increased sensitivity and specificity, minimizing the need for cumbersome retesting, even in the most difficult matrices.


Ordering Information

Atlas E. coli O157:H7 EG2 Detection Assay
250 tests per kit
Catalog #PRT-01252

Orders (or general questions about ordering): Customer Support
Product-related questions: Technical Support


1. Centers for Disease Control and Prevention. E. coli (Escherichia coli).

011401 AOAC_Ecoli

The Atlas E. coli O157:H7 EG2 Detection Assay has been certified by the AOAC Research Institute Performance Tested MethodsSM program and assigned PTM certification #011401.

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